Solvent:Article Title: Boosting the Biosynthesis of Ursodeoxycholic Acid via a Novel 7β-Hydroxysteroid Dehydrogenase from <i>Olsenella</i> sp. and Biotransformation Systematic Optimization
Article Snippet: .. 1 ± 3.3 158 ± 3.8 This study Free enzyme 7-HSDH from Xanthomo nas maltophili a CBS 897.97 7α-HSDH from Xanthomo nas maltophili a CBS 897.97, - 2-hexanol (50 mM) CDCA 10 - - - - 82 0.02 17 S12 NAD+ Free enzyme, one-pot reaction 7β-HSDH from Ruminococ cus torques ATCC 35915 7α-HSDH from Clostridiu m absonum, NADP+ - - CDCA 10 - - - - 73 29 9 Free enzyme, two-step reaction 7β-HSDH from Ruminococ cus torques 7α-HSDH from Clostridiu m absonum, - Glucose (30 mM) and sodium pyruvate (30 mM) CDCA 10 10 0 98.3 ± 2.4 - - 98 118 9 S13 ATCC 35915 NADP+ Free enzyme, two-step reaction 7β-HSDH from Ruminococ cus torques ATCC 35915 7α-HSDH from Escherichi a coli, NAD+ - Glucose (150 mM) and sodium pyruvate (150 mM) CDCA 100 10 0 80 - - 80 141 23 Immobilized Clostridium absonum cells - - - - CDCA 0.5 - - - - 80 0.2 29 S14 Free enzyme, two-step reaction 7β-HSDH from C. absonum 7α-HSDH from C. absonum, NADP+ - Glucose (50 mM) and dithiothreitol (1 mM) CDCA 12.5 - - - - 86 2.1 30 Free enzyme, two-step reaction 7β-HSDH from R. torques ATCC3591 5 7α-HSDH from C. absonum DSM599, NADP+ - CDCA 50.9 - - - - 90 18 31 Chemoenzy matic transformati ons, 7β-HSDH from Collinsella aerofacien - 3α-HSDH from Comamon as Sodium formate (600 mM), and Wolff–Kishner reduction Dehydroch olic acid (DHCA) 72 97 94.8 - - - - 32 S15 three-step reaction s testostero ni, NAD+ chemicals (e.g., 85% (w/v) KOH, 80% (w/v) hydrazinium hydroxide, 81.4% (v/v) ethylene glycol monomethylether, acidified to pH=2 with 40% H2SO4) Chemoenzy matic transformati - 7α-HSDH from Acinetoba 12α-HSD H from A. calcoaceti 85% (w/v) KOH, 80% (w/v) hydrazinium Cholic acid (sodium cholate) 12 98 90 97 82 70 - 12 S16 ons, four-step reaction cter calcoaceti cus lwoffii, NAD+ cus lwoffii, NAD+ hydroxide, 81.4% (v/v) ethylene glycol monomethylether, acidified to pH=2 with 40% H2SO4, 10% (w/v) sodium, anhydrous 2-butanol (solvent during reduction of 7-ketochenodeoxy cholic acid), and S17 sodium pyruvate (60 mM) 6 a, 100 g·L-1 CDCA equal to 254.7 mM; -, no report. .. 1 ± 3.3 158 ± 3.8 This study Free enzyme 7-HSDH from Xanthomo nas maltophili a CBS 897.97 7α-HSDH from Xanthomo nas maltophili a CBS 897.97, - 2-hexanol (50 mM) CDCA 10 - - - - 82 0.02 17 S12 NAD+ Free enzyme, one-pot reaction 7β-HSDH from Ruminococ cus torques ATCC 35915 7α-HSDH from Clostridiu m absonum, NADP+ - - CDCA 10 - - - - 73 29 9 Free enzyme, two-step reaction 7β-HSDH from Ruminococ cus torques 7α-HSDH from Clostridiu m absonum, - Glucose (30 mM) and sodium pyruvate (30 mM) CDCA 10 10 0 98.3 ± 2.4 - - 98 118 9 S13 ATCC 35915 NADP+ Free enzyme, two-step reaction 7β-HSDH from Ruminococ cus torques ATCC 35915 7α-HSDH from Escherichi a coli, NAD+ - Glucose (150 mM) and sodium pyruvate (150 mM) CDCA 100 10 0 80 - - 80 141 23 Immobilized Clostridium absonum cells - - - - CDCA 0.5 - - - - 80 0.2 29 S14 Free enzyme, two-step reaction 7β-HSDH from C. absonum 7α-HSDH from C. absonum, NADP+ - Glucose (50 mM) and dithiothreitol (1 mM) CDCA 12.5 - - - - 86 2.1 30 Free enzyme, two-step reaction 7β-HSDH from R. torques ATCC3591 5 7α-HSDH from C. absonum DSM599, NADP+ - CDCA 50.9 - - - - 90 18 31 Chemoenzy matic transformati ons, 7β-HSDH from Collinsella aerofacien - 3α-HSDH from Comamon as Sodium formate (600 mM), and Wolff–Kishner reduction Dehydroch olic acid (DHCA) 72 97 94.8 - - - - 32 S15 three-step reaction s testostero ni, NAD+ chemicals (e.g., 85% (w/v) KOH, 80% (w/v) hydrazinium hydroxide, 81.4% (v/v) ethylene glycol monomethylether, acidified to pH=2 with 40% H2SO4) Chemoenzy matic transformati - 7α-HSDH from Acinetoba 12α-HSD H from A. calcoaceti 85% (w/v) KOH, 80% (w/v) hydrazinium Cholic acid (sodium cholate) 12 98 90 97 82 70 - 12 S16 ons, four-step reaction cter calcoaceti cus lwoffii, NAD+ cus lwoffii, NAD+ hydroxide, 81.4% (v/v) ethylene glycol monomethylether, acidified to pH=2 with 40% H2SO4, 10% (w/v) sodium, anhydrous 2-butanol (solvent during reduction of 7-ketochenodeoxy cholic acid), and S17 sodium pyruvate (60 mM) 6 a, 100 g·L-1 CDCA equal to 254.7 mM; -, no report.
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